IASR Journal of Agriculture and Life Sciences
2022, Volume 2, Issue 1 : 11-29
Research Article
Morphological characterization of sorghum [Sorghum bicolor (L.) Moench] land race collected from western and south-western part of Ethiopia for DUS Traits
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Received
March 24, 2022
Accepted
April 20, 2022
Published
May 27, 2022
Abstract

A total of 83-sorghum landraces collected from the western and south-western parts of Ethiopia were characterized using 22 morphological descriptors provided by PPV and FRA for DUS testing in sorghum. The aim of this research is to characterize Sorghum landraces morphologically for DUS traits collected from south-western Ethiopia. Results revealed diverse characters among the traits and the tested sorghum land races. The highest group was classified into 11 groups based on inflorescence compactness. The second diverse group was obtained by based on glume color traitssorghum landraces have six groups. The result divided the panicle exertion into five groups, the endosperm texture into five groups, the waxy bloom into four groups, the glume cover into three groups, and the leaf mid color into three groups. observed by each of the traits, endosperm color, grain luster, and grain form. The lowest (1 group) was characterized by stalk juiciness. All 83 (100%) tested landraces did not show the character of juiciness. Classification of genotypes based on DUS traits provided identification of key characteristics of various genotypes. Thus, the present clearly indicates the utilization of the PPV and FRA descriptors for the purpose of registration, maintenance, and protection of the collected sorghum land race.

Keywords
INTRODUCTION

The monocotyledon crop sorghum [Sorghum bicolor (L.) Moench] belongs to the Gramineae family. It is a naturally self-pollinated short-day plant with up to 30% spontaneous crosspollination depending on panicle kinds[1].Sorghums, both wild and cultivated, are diploid (2n = 2x = 20) and tropical in origin. It thrives in areas between the equator's 400N and 400S latitudes[2]. Sorghum is grown in both tropical and temperate settings, but it is most recognized for its tolerance to the world's semi-arid tropical (SAT) regions, which are prone to drought [1].

 

Sorghum is a staple food for more than 500 million people in 30 African and Asian countries. [3]. Sorghum is important for food security in Africa because of its drought tolerance and ability to withstand high temperatures and water logging. Because of its resistance to drought and other production constraints, sorghum is a key food and nutritional security crop for more than 100 million people in Eastern horn of Africa[4].

 

In Ethiopia, approximately 74% of total sorghum grain output is consumed at the household level, with the remaining being sold and used for seed at the local level. The grain is used to make a variety of local staple foods such as leavened bread (injera), porridge, and local beverages, all of which require specific grain quality characteristics. Additionally, the Stover, which is used for animal feed, fuel, and the construction of fences and shelters, is often valued as highly as grain yield, so farmers prefer taller varieties[5].                                                                                                                 

Planting of cereals Before the 1950s, it was made up of landraces, or local farmer's variety [6]. Landrace refers to a cultivated plant that has an unknown origin, is locally/environmentally suited, or has not undergone a rigorous breeding effort[7].Farmers have maintained and cultivated landrace variations for decades using a traditional method of selection. In a low-input agricultural system, an autochthonous landrace is a variety with a high capacity to endure biotic and a biotic stress, resulting in high yield stability and an intermediate yield level [8].

 

Ethiopia is known as one of the Vavilovian centers of origin, or diversification, for a variety of cultivated and wild crop species, including sorghum [9].Ethiopia has been identified as a center of origin and/or diversification for a number of grain crops, including sorghum. [10].

 

Sorghum is one of the most widely grown grains in the world. Over 95% of sorghum fields in Ethiopia are planted to landraces, and over 95% of the sorghum types farmed are farmer-selected landraces [11]. The wild (Sorghum bicolor subsp. arundinaceum) and cultivated sorghums are more likely to exchange genetic material since they both grow in sympathy with their wild and weedy cousins in most sorghum-growing areas of Ethiopia, primarily in the south-eastern and south-western regions [2]. Within a species, there was more genetic diversity, which was often taken as a measure of its ability to adapt to its new environment. As a result, biodiversity is a valuable resource for coping with environmental changes. Sorghum is one of the world's largest crop germplasm collections, with over 42,000 accessions[12,13].

 

The physical and genetic identification and classification of germplasm is made possible by characterization. Morphological characterization allows for the creation of a catalog of descriptors that are necessary for collection management or agricultural application[14]. Morphological characterization allows for the creation of a catalog of descriptors that are necessary for collection management or agricultural application[15]Therefore, a greater understanding of the genetic variety in sorghum crop species will undoubtedly aid in the morphological characterization of Sorghum germplasm for DUS features, as well as the future enhancement of this sorghum genetic architecture.

 

MATERIALS AND METHODS

The experiment was conducted at Assosa Agricultural Research Center on main growing season of 2017/18. Assosa Agricultural Research Center (AsARC) is located in the Benishangul-Gumuz Regional State at western part of Ethiopia which is 650 km far away from Addis Abeba and also its altitude 1547.The experimental material consists of 83 they have been maintained by farming community and research station and the experiment was planted in a randomized block design with three replications. The lines were grown in the 5 meter length with 75 cm (row to row) and the 15 cm (plant to plant) distance. All plots management practices were applied as per recommendation.

 

Data collection was made by randomly selecting and tagging 20 individual plants and a total of 22 traits such as leaf midrib color, waxy bloom ,Plant height up to base of flag leaf, Grain covering, glume color, Head compactness and shape, stalk juiciness, Inflorescence exertion, Tillering, Lodging, Over all plant aspect Shattering, grain size, endosperm color, Endosperm type, Endosperm texture, Gain plumpness, grain shape(profile view), Caryopsis/grain color, grain luster, Grain form and Threshebility was used for  morphological characterization of landraces on the basis of DUS guidelines.

 

The accession used for the present study is listed in Table 1. The data was taken on the basis of DUS guidelines for Sorghum Distinctiveness for the traits were also checked for the all the genotypes. The descriptors and the time and way of taking data are described in Table 2. Grouping of sorghum germplasm was done by utilizing grouping characteristics which mentioned in the DUS test guidelines.

 

RESULT AND DISCUSSION

5th leaf stage

The color of the leaf midrib was used to differentiate genotypes at the fifth leaf stage. This character's genotypes were divided into three groups: white 27.71% (23 genotypes), yellow 68.67% (57 genotypes), and green 3.61% (3).These findings are consistent with those of[16,17].

 

Vegetative stage             

Waxy bloom, which is a waxy coating on the surface of the internode that is somewhat present 42.16% (35 genotypes), medium48.19%  (40 genotypes), substantially bloomy 8.43% (7genotypes), and entirely bloomy8.43% (7 genotypes) were used to define genotypes at the vegetative state 2.4%(2 genotypes). It has been experimentally proven that this waxy layer significantly reduces transpiration (20 to 30%), [18].It prevents water from clogging the stomata and preventing the passage of gases, according to [19].It also aids in the prevention of harm to the stem, blooms, and fruits by ants and other crawling insects. In regard to the process of production of the waxy covering, [18]has noted that that the wax did not come from chemical modification of the cuticle or other layers of the outer wall, as could be expected, but rather was a product of secretion.

 

Physiological Maturity stage

At maturity stage genotypes were characterized on the basis of five character the amount of grain covered by 25% glume cover computed 57.83% (48 genotypes),50% glume covered scored 32.53% (27genotypes) 75 and 100% glume cover 4.82%  scored (4 genotypes).The second character was Glume color white1.20% (1genotypes),2.40% sienna(2 genotypes),20.48% red 36.14%(17genotypes) purple 40.96%( 30 genotypes) and grey (34 genotype)(Table.2).

 

Genotypes were classified into eleven categories based on inflorescence compactness. Panicle is exceedingly flexible (typical of wild sorghum has scored 0.24% 2 genotypes), and primary branches are very loose and drooping 9.63% ( 8 genotypes)  7.22% (6 genotypes) loose erect primary branch, loose drooping primary branch 22.89 % (19 genotypes) Semi-loose erect primary branches 8.4% (7 genotypes), semi-loose drooping primary branches 19.27% (16 genotypes), semi-compact18.07% (15 genotypes), compact elliptic 10.8% (9 genotypes), compact oval 6.02%(5 genotypes),and half-broom corn 15.66% (13 genotypes).The number and growth of branches, or inflorescence architecture, is very important in this family because it directly affects s grain yield [20].There is no genotypes exhibit Stalk juiciness based on Stalk juiciness traits.

 

Genotypes were classified into four groups based on Panicle exertion: slightly exerted (2 cm but ligule of hag leaf definitely below inflorescence base16.86% (14 genotypes), (2-10cm between ligule and inflorescence base 36.14% (30 genotypes), well exerted (> 10 cm between ligule and inflorescence base39.74% (33 genotypes), and peduncle recurved (inflorescence below ligule and clearly exposed splitting 7.22 % (6 genotypes).

 

The genotypes were divided into three categories based on their threshability. The following genotypes were investigated: Very low83.13% (69 genotypes), low threshability1.20% (1 genotype), intermediate threshability10.84% (9 genotypes), and very high threshability4.81% (4 genotypes).The ease with which grains may be separated from panicles and glumes during threshing is a critical factor in sorghum utilization, especially in smallholder sorghum production when threshing is done by hand[21]. Some sorghum cultivars have grains that are securely connected to the panicle, making threshing difficult. As a result, a large portion of the grain is either rejected with the chaff, resulting in considerable postharvest losses, or gathered with the glumes still attached, resulting in significant quality and market value drop as the grains become less desirable to customers [21].

 

Based on the grain size genotypes of small size are 8.43% (7 genotypes), middle33.73% (28 genotypes), and large 57.83% (48 genotypes) sizes were identified. landraces were divided into two groups based on the endosperm color character: landraces with red color7.22% (6 genotypes) and genotypes with white color92.77% (77 genotypes). Based on endosperm type (47 genotypes) were normal types whereas 36 genotypes are waxy type 36 Variation in grain size, a major determinant of grain yield and quality in cereal crops, is determined by both the plant’s genetic potential and the available assimilate to fill the grain in the absence of stress [22].

 

The genotypes were divided into five groups based on endosperm texture. There were 5 entirely corneous genotypes, 7 largely corneous genotypes, and 27 intermediate genotypes,23 genotypes that were largely starch and 21 genotypes that were fully starch. The genotypes were divided into two groups based on the grain plumpness trait. The number of dimple genotypes was 43, while the number of fat genotypes was 40. Based on Grain profile genotypes with narrow elliptic (8 genotypes), elliptic (25 genotypes), and 50 genotypes circular based on Grain profile. Based on various Caryopsis grain colors white 53.01(44 genotypes), yellow 30.12% (25 genotypes), red 6.02% (5 genotypes) and 10.84% (9 genotypes).

 

Grain luster are scored (75 genotypes) and not lustrous computed (8genotypes). Grain form twins (2 genotypes) and singles (81 genotypes).On the basis of tillering and lodging, 83 genotypes were found to have neither tillering nor lodging. On the basis of shattering genotypes, 2 groups were identified: 47 genotypes with a high level of shattering character and 36 genotypes with a low level of shattering character. Genotypes were classified into four groups based on plant agronomic aspects, with genotypes with high scores being placed in the first group (15 genotypes) There were 32 genotypes with an average score of 32, 24 genotypes with a below average score of 24, and 12 genotypes with a poor score of 12.

 

CONCULUSION

The present study shows that morphological description of 83-sorghum landraces based on the DUS descriptor can be effectively used for identification and grouping of sorghum landraces for further breeding programs. The results reveal that there is diverse character among the tested traits, Panicle shape is calculated as the highest group (11 groups), in contrast to stalk juiciness, which has the lowest (1 group), which means 83 (100%) of the tested landraces do not have the characteristics of  juiciness. Using satisfied DUS criteria for all of these morphological descriptors protects the rights of plant breeders and farmers. Even though these morphological descriptors alone may not be sufficient for DUS criteria

Acknowledgments

The authors would like thank all staff of Assosa Agricultural Research center (AsARC) particularly crop research process.

 

Conflicts of interest

There are no potential conflicts of interest

 

Table.1 List of Accession used for study

Plot#

Accession no

Plot#

Accession no

Plot#

Accession no

Plot#

Accession no

Plot#

Accession no

1

NJ 001

18

Ka018

35

Ya035

52

Ag055

69

Mok074

2

NJ 002

19

Ka019

36

Ya036

53

Bab056

70

Bam075

3

NJ 003

20

Ka020

37

Ya037

54

Bab057

71

Mok076

4

Boj004

21

Ka021

38

Ya038

55

Bab058

72

Mok079

5

Boj005

22

Ka022

39

ya039

56

Bab059

73

Mok081

6

Boj006

23

Ka023

40

Ya040

57

Ba060

74

Mok085

7

Boj007

24

Ka024

41

ya041

58

Ba061

75

Mok086

8

Boj008

25

Ka025

42

Ya042

59

Bab062

76

Bmb097

9

Boj009

26

Ka026

43

Ag044

60

Bab063

77

Bmb102

10

Ka010

27

Ka027

44

Ag045

61

Bab064

78

Rb109

11

Ka011

28

Ka028

45

Ag046

62

Ba065

79

Rb111

12

Ka012

29

Ya029

46

Ag047

63

Ba066

80

Ba118

13

Ka013

30

Ya030

47

Ag048

64

Ba067

81

Ba119

14

Ka014

31

Ya031

48

Ag049

65

Qon070

82

Ba120

15

Ka015

32

Ya032

49

Ag052

66

Qonn071

83

Ba121

16

Ka016

33

Ya033

50

Ag053

67

Qon072

 

 

17

Ka017

34

Ya034

51

Ag054

68

Qon073

 

 

 

 

Table.2 Descriptors used to characterize the present genotypes

No.

Parameters

States

Developmental stage

2.1

leaf midrib color

 

Yellow

Green

White

 

5thleaf stage

2.2

waxy bloom

 

Slightly present

Medium

Mostly bloom

Completely bloom

 

Vegetative

2.3

Plant height up to base of flag leaf

Very short(<76cm)
short (76-150 cm)
medium (151-225 cm)
tall (226-300 cm)
very tall (>300 cm)

Panicle emergency

2.4

Grain covering

 

25% grain covering

50% grain covering

75%grain covering

Grain fully covered

Glumes longer than grain

Physiological maturity

2.5

glume color

 

White(whitegroup155D,155C,155B,155A)

Physiological maturity

2.6

Head compactness and shape

 

Very lax panicle(typical of wild sorghum)

Very loose erect primary branches

Loose erect primary branch

Loose drooping Primary branch

Semi-loose erect primary branch

Semi compact elliptic

Compact elliptic

Compact oval

Half broom corn

Broom corn

Others( not specify)

 

Physiological maturity

2.7

stalk juiciness

 

Juicy

Not juicy

Physiological maturity

2.8

Inflorescence exertion

 

 

Flowering

2.9

Tillering

Absent

Present

Physiological maturity

2.10

Lodging

Low

Intermediate

High

Physiological maturity

2.11

Over all plant aspect

Very good

good

average

below average

 poor

 

Physiological maturity

2.12

Shattering

Very low

Low

Intermediate

High

Very high

Physiological maturity

2.13

grain size

 

Small

Medium

Large

After threshing

2.14

endosperm color

 

White

Yellow

After threshing

2.15

Endosperm type

 

Normal

Waxy

Sugar

After threshing

2.16

Endosperm texture

 

Completely corneous

Mostly corneous

intermediate

Mostly starch

Completely starchy

After threshing

2.17

Gain plumpness

 

Dimple

Plump

After threshing

2.18

grain shape(profile view)

 

Narrow elliptic
elliptic
circular

After threshing

2.19

Caryopsis/grain color

 

White(White group 155D 155C 155B 155A)

Yellow (Yellow group 6 D 6C 6B 6A)

Red(orange-Red- group164 B164 A164B164 A

Brown(Browngroup33A33C33B33A33B33A)

Buff greyed orange group(166B)

Other

After threshing

2.20

grain luster

 

Absent

Present

After threshing

2.21

Grain form

 

Single

Twins

After threshing

2.22

Threshebility

Freely threshable

Partly threshable

Difficult to thresh

After threshing

 

 

Accession no

Leaf midrib color

Waxy bloom

Plant height up to base of flag leaf

Grain covering

Glume color

Head compactness and shape

Stalk juiciness

Inflorescence exertion

Tillering

Lodging susceptible

Plant aspect

 
 

NJ 001

White

Slightly present

Very tall

75% grain covered

Black

Semi loose drooping primary branches

Not juicy

Peduncle recurved

Absent

Low

Average

 

NJ 002

White

Slightly present

Very tall

25%  grain covered

Grey

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

NJ 003

Yellow

Mostly bloom

Tall

25%  grain covered

Grey

Compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Boj004

Yellow

Slightly present

Very tall

Grain fully covered

Grey

Half broom corn

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Boj005

White

Medium

Very tall

50% grain covered

Grey

Very lax panicle

Not juicy

Slightly exerted<2cm

Absent

Low

Poor

 

Boj006

White

Slightly present

Very tall

25%  grain covered

Grey

Semi loose erect  primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Boj007

Yellow

Medium

Very tall

25%  grain covered

Black

Compact oval

Not juicy

Peduncle recurved

Absent

Low

Average

 

Boj008

Yellow

Slightly present

Very tall

25%  grain covered

Black

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Boj009

White

Slightly present

Very tall

25%  grain covered

Black

Loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

Ka010

Yellow

Slightly present

Very tall

25%  grain covered

Black

Half broom corn

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

Ka011

Yellow

Medium

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ka012

White

Slightly present

Very tall

25%  grain covered

Black

Compact oval

Not juicy

Peduncle recurved

Absent

Low

Average

 

Ka013

Yellow

Slightly present

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka014

Yellow

Medium

Very tall

25%  grain covered

Red

Semi loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ka015

Yellow

Slightly present

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ka016

Yellow

Slightly present

Very tall

25%  grain covered

Purple

Loose erect primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ka017

Yellow

Medium

Very tall

25%  grain covered

Black

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ka018

Yellow

Slightly present

Tall

25%  grain covered

Purple

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka019

Yellow

Slightly present

Very tall

25%  grain covered

Purple

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

Ka020

Yellow

Medium

Very tall

25%  grain covered

Purple

Very lax panicle

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

Ka021

Yellow

Medium

Very tall

25%  grain covered

Grey

Loose erect primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ka022

White

Medium

Very tall

50% grain covered

Grey

Loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka023

Yellow

Medium

Very tall

25%  grain covered

Black

Half broom corn

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ka024

Yellow

Medium

Very tall

25%  grain covered

Black

Half broom corn

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka025

Yellow

Slightly present

Very tall

50% grain covered

Black

Loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka026

Yellow

Medium

Very tall

25%  grain covered

Purple

Semi loose erect  primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ka027

Yellow

Medium

Very tall

50% grain covered

Purple

Loose erect primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ka028

Yellow

Slightly present

Very tall

50% grain covered

Purple

Loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ya029

Yellow

Medium

Very tall

25%  grain covered

Grey

Loose erect primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ya030

Yellow

Medium

Very tall

50% grain covered

Purple

Loose erect primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ya031

Yellow

Slightly present

Very tall

50% grain covered

Black

Loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ya032

Yellow

Medium

Very tall

25%  grain covered

Black

Half broom corn

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ya033

Yellow

Medium

Very tall

25%  grain covered

Black

Compact oval

Not juicy

Peduncle recurved

Absent

Low

Good

 

Ya034

Yellow

Medium

Very tall

50% grain covered

Grey

Loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ya035

Yellow

Medium

Very tall

50% grain covered

Grey

Semi loose erect  primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Poor

 

Ya036

Yellow

Mostly bloom

Tall

25%  grain covered

Grey

Compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ya037

Yellow

Completely bloom

Tall

25%  grain covered

Black

Compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ya038

Yellow

Medium

Very tall

25%  grain covered

Black

Compact oval

Not juicy

Peduncle recurved

Absent

Low

Good

 

ya039

Yellow

Medium

Very tall

50% grain covered

Grey

Half broom corn

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ya040

Yellow

Slightly present

Very tall

Grain fully covered

Purple

Loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

ya041

Yellow

Slightly present

Very tall

25%  grain covered

Purple

Very loose  erect primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ya042

White

Medium

Very tall

25%  grain covered

Grey

Semi compact elliptic

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ag044

White

Slightly present

Very tall

75% grain covered

Purple

Loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Ag045

White

Slightly present

Very tall

25%  grain covered

Black

Loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ag046

White

Slightly present

Very tall

75% grain covered

Black

Semi loose erect  primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Ag047

Yellow

Slightly present

Tall

25%  grain covered

Purple

Loose erect primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ag048

Yellow

Medium

Very tall

25%  grain covered

Grey

Half broom corn

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Ag049

Yellow

Medium

Very tall

50% grain covered

Grey

Compact oval

Not juicy

Peduncle recurved

Absent

Low

Below average

 

Ag052

Yellow

Medium

Very tall

50% grain covered

Purple

Half broom corn

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ag053

White

Slightly present

Very tall

50% grain covered

Black

Loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Ag054

White

Slightly present

Very tall

50% grain covered

Purple

Loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Ag055

Yellow

Slightly present

Very tall

75% grain covered

Grey

Loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Bab056

White

Slightly present

Very tall

50% grain covered

Purple

Loose erect primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Bab057

White

Medium

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Bab058

Yellow

Medium

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Bab059

Yellow

Medium

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ba060

Yellow

Slightly present

Very tall

25%  grain covered

Purple

Semi loose erect  primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Ba061

White

Slightly present

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Bab062

White

Slightly present

Very tall

25%  grain covered

Black

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Bab063

Yellow

Medium

Tall

25%  grain covered

Grey

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Bab064

Yellow

Medium

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Ba065

Yellow

Completely Bloom

Medium

50% grain covered

Grey

Compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Ba066

Yellow

Medium

Very tall

25%  grain covered

Purple

Compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Ba067

Yellow

Medium

Very tall

25%  grain covered

Purple

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Poor

 

Man068

Yellow

Medium

Very tall

25%  grain covered

Purple

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Poor

 

Qon070

Yellow

Medium

Very tall

25%  grain covered

Grey

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Qonn071

Yellow

Slightly present

Very tall

25%  grain covered

Grey

Compact elliptic

Not juicy

Slightly exerted<2cm

Absent

Low

Average

 

Qon072

White

Medium

Very tall

75% grain covered

Black

Semi compact elliptic

Not juicy

Slightly exerted<2cm

Absent

Low

Good

 

Qon073

Yellow

Medium

Tall

75% grain covered

Grey

Very loose drooping primary branches

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Mok074

Yellow

Slightly present

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Average

 

Bam075

Yellow

Medium

Very tall

25%  grain covered

Grey

Semi compact elliptic

Not juicy

Well exerted> 10cm

Absent

Low

Good

 

Mok076

Yellow

Slightly present

Very tall

50% grain covered

Black

Semi loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Poor

 

Mok079

Yellow

Slightly present

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Good

 

Mok081

Yellow

Medium

Very tall

50% grain covered

White

Half broom corn

Not juicy

Exerted 2-10cm

Absent

Low

Below average

 

Mok085

White

Mostly bloom

Tall

25%  grain covered

Grey

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Mok086

White

Mostly bloom

Very tall

50% grain covered

Grey

Semi loose drooping primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Bmb097

Yellow

Medium

Very tall

50% grain covered

Grey

Semi compact elliptic

Not juicy

Slightly exerted<2cm

Absent

Low

Good

 

Bmb102

Yellow

Medium

Very tall

50% grain covered

Purple

Semi loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Good

 

Rb109

Yellow

Mostly bloom

Medium

50% grain covered

Black

Semi compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Rb111

White

Slightly present

Very tall

75% grain covered

Red

Semi loose erect  primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ba118

Green

Slightly present

Very tall

25%  grain covered

Black

Semi loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ba119

White

Medium

Very tall

75% grain covered

Grey

Very loose drooping primary branches

Not juicy

Well exerted> 10cm

Absent

Low

Below average

 

Ba120

White

Mostly bloom

Medium

25%  grain covered

Grey

Semi compact elliptic

Not juicy

Slightly exerted<2cm

Absent

Low

Below average

 

Ba121

Yellow

Medium

Very tall

Grain fully covered

Black

Semi loose erect  primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Average

 

Assosa-1

White

Medium

Medium

25%  grain covered

Grey

Semi loose erect  primary branches

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

Adukara

Yellow

Medium

Medium

25%  grain covered

Purple

Compact elliptic

Not juicy

Exerted 2-10cm

Absent

Low

Good

 

 

Accession no

Shattering

grain size

endosperm color

Endosperm type

Endosperm texture

Gain plumpness

grain shape(profile view

Caryopsis/grain color

grain luster

Grain form

Threshebility

 
 

NJ 001

Very low

medium

white

Sugar

Mostly starch

Plump

Elliptic

Brown

Absent

single

Freely threshable

 

NJ 002

Very low

medium

white

Normal

Intermediate

Plump

Narrow elliptic

Brown

Absent

Single

Partly threshable

 

NJ 003

Very low

Large

white

Normal

Mostly starch

Dimple

Circular

Red

Absent

Single

Freely threshable

 

Boj004

Very low

Medium

white

Sugar

Completely starch

Plump

Elliptic

Brown

Absent

Single

Partly threshable

 

Boj005

Very low

large

White

Normal

Intermediate

Dimple

Circular

White

Present

Single

Freely threshable

 

Boj006

Very low

Medium

White

Normal

Intermediate

Plump

Elliptic

White

Absent

Single

Difficult threshable

 

Boj007

Very low

Medium

White

waxy

Completely corneous

Plump

circular

White

Absent

Single

Freely threshable

 

Boj008

Low

large

White

sugar

Mostly starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Boj009

Very low

large

White

waxy

Intermediate

Plump

circular

Red

Absent

Single

Freely threshable

 

Ka010

Very low

Large

White

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Ka011

Low

Large

White

sugar

Mostly starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka012

Very low

Large

White

waxy

Mostly starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka013

Very low

Large

White

sugar

Completely starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka014

Low

medium

White

waxy

Intermediate

Dimple

circular

White

present

Single

Freely threshable

 

Ka015

Very low

large

White

sugar

Completely starch

Dimple

circular

Red

Absent

Single

Freely threshable

 

Ka016

Very low

medium

White

sugar

Completely starch

Dimple

elliptic

White

Present

Single

Freely threshable

 

Ka017

Very low

Large

White

Sugar

Completely starch

Dimple

circular

Red

Absent

Single

Freely threshable

 

Ka018

Very low

Large

White

waxy

Intermediate

Dimple

circular

Red

Absent

Single

Freely threshable

 

Ka019

Very low

Large

White

sugar

Completely starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka020

Very low

Large

White

sugar

Mostly starch

Dimple

circular

White

Absent

Twins

Freely threshable

 

Ka021

Very low

Large

White

sugar

Completely starch

Dimple

circular

White

Absent

Twins

Freely threshable

 

Ka022

Very low

Large

White

Waxy

Completely starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka023

Very low

Large

White

sugar

Completely starch

Dimple

circular

Red

Absent

Single

Freely threshable

 

Ka024

Very low

Large

White

waxy

Intermediate

Dimple

circular

White

Absent

Single

Freely threshable

 

Ka025

Very low

Large

White

sugar

Completely starch

Dimple

circular

Other

Absent

Single

Freely threshable

 

Ka026

Very low

Large

White

sugar

Intermediate

Dimple

circular

Other

Absent

Single

Freely threshable

 

Ka027

Very low

Large

White

waxy

Mostly corneous

Dimple

elliptic

Other

Absent

Single

Freely threshable

 

Ka028

Very low

Large

White

waxy

Mostly corneous

Plump

circular

Red

Absent

Single

Freely threshable

 

Ya029

Very low

medium

White

waxy

Intermediate

Plump

circular

White

Absent

Single

Freely threshable

 

Ya030

Very low

Large

White

Waxy

Intermediate

Dimple

circular

White

Absent

Single

Freely threshable

 

Ya031

Very low

Large

White

sugar

Completely starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Ya032

Very low

Large

White

sugar

Completely starch

Dimple

elliptic

Red

Absent

Single

Freely threshable

 

Ya033

Very low

medium

White

Sugar

Intermediate

Plump

elliptic

White

Absent

Single

Freely threshable

 

Ya034

Very low

Large

White

sugar

Mostly starch

Plump

elliptic

Red

Absent

Single

Freely threshable

 

Ya035

Very low

Large

White

sugar

Mostly starch

Dimple

circular

Red

Absent

Single

Freely threshable

 

Ya036

Very low

Large

White

Sugar

Mostly starch

Plump

circular

Other

Absent

Single

Freely threshable

 

Ya037

Very low

Medium

White

Sugar

Mostly starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Ya038

Very low

Large

White

Waxy

Mostly corneous

Dimple

circular

White

Absent

Single

Freely threshable

 

ya039

Very low

Medium

White

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Ya040

Very low

large

White

waxy

Intermediate

Dimple

circular

Red

Absent

Single

Freely threshable

 

ya041

Very low

Large

White

Waxy

Intermediate

Dimple

circular

White

present

Single

Freely threshable

 

Ya042

Very low

medium

White

waxy

Intermediate

Dimple

circular

Other

Absent

Single

Freely threshable

 

Ag044

Very low

Large

White

Sugar

Mostly starch

Dimple

elliptic

Red

Absent

Single

Freely threshable

 

Ag045

Very low

Large

White

Sugar

Intermediate

Plump

circular

Red

Absent

Single

Freely threshable

 

Ag046

Very low

Small

White

waxy

Completely corneous

Plump

Narrow elliptic

White

Absent

Single

Freely threshable

 

Ag047

Very low

medium

Yellow

Sugar

Completely starch

Plump

elliptic

Brown

Absent

Single

Freely threshable

 

Ag048

Very low

Large

White

Waxy

Completely corneous

Dimple

circular

White

Absent

Single

Freely threshable

 

Ag049

Very low

medium

White

sugar

Completely starch

Plump

circular

White

Absent

Single

Freely threshable

 

Ag052

Very low

Large

White

sugar

Completely starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ag053

Very low

Large

White

sugar

Mostly starch

Plump

Narrow elliptic

White

Absent

Single

Freely threshable

 

Ag054

Very low

Medium

Yellow

waxy

Completely corneous

Plump

Narrow elliptic

Other

Absent

Single

Partly threshable

 

Ag055

Very low

small

white

waxy

Mostly corneous

Plump

Narrow elliptic

White

Absent

Single

Difficult threshable

 

Bab056

Very low

Small

yellow

waxy

Intermediate

Plump

circular

White

Absent

Single

Difficult threshable

 

Bab057

Very low

Medium

White

Sugar

Mostly starch

Dimple

circular

White

Absent

Single

Partly threshable

 

Bab058

Very low

medium

White

waxy

Mostly corneous

Dimple

Elliptic

Other

Absent

Single

Partly threshable

 

Bab059

Very low

Medium

White

Sugar

Completely starch

Plump

circular

White

Absent

Single

Freely threshable

 

Ba060

Very low

Large

White

sugar

Mostly starch

Dimple

Elliptic

White

present

Single

Freely threshable

 

Ba061

Very low

Medium

White

Sugar

Completely starch

Dimple

Elliptic

Red

Absent

Single

Freely threshable

 

Bab062

Very low

medium

White

Sugar

Completely starch

Dimple

Narrow elliptic

Red

Absent

Single

Freely threshable

 

Bab063

Very low

medium

yellow

sugar

Mostly starch

Dimple

circular

Other

Absent

Single

Freely threshable

 

Bab064

Very low

Large

White

sugar

Completely starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Ba065

Very low

medium

white

sugar

Intermediate

Plump

circular

White

Absent

Single

Freely threshable

 

Ba066

Very low

Large

White

Sugar

Completely starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Ba067

Very low

Large

yellow

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Man068

Very low

Medium

yellow

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Qon070

Very low

Large

White

Sugar

Mostly starch

Dimple

circular

White

Absent

Single

Freely threshable

 

Qonn071

Very low

Large

White

sugar

Mostly starch

Dimple

Elliptic

Red

Absent

Single

Freely threshable

 

Qon072

Very low

Large

White

waxy

intermediate

Dimple

elliptic

Red

Absent

Single

Freely threshable

 

Qon073

Very low

small

White

waxy

Mostly corneous

Plump

Narrow elliptic

White

present

Single

Partly threshable

 

Mok074

Very low

Medium

White

sugar

Intermediate

Plump

circular

White

Absent

Single

Freely threshable

 

Bam075

Very low

Large

White

Sugar

Mostly starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Mok076

Very low

Medium

White

waxy

Intermediate

Dimple

circular

Other

Absent

Single

Freely threshable

 

Mok079

Very low

medium

White

Waxy

Intermediate

Plump

circular

White

Absent

Single

Freely threshable

 

Mok081

Very low

small

White

waxy

Mostly starch

Plump

elliptic

White

present

Single

Freely threshable

 

Mok085

Very low

Large

White

Sugar

Completely starch

Dimple

elliptic

Red

present

Single

Freely threshable

 

Mok086

Very low

medium

White

waxy

intermediate

Plump

circular

White

Absent

Single

Freely threshable

 

Bmb097

Very low

Large

White

sugar

Mostly starch

Plump

circular

Red

Absent

Single

Freely threshable

 

Bmb102

Very low

Medium

White

Waxy

Intermediate

Plump

elliptic

White

Absent

Single

Freely threshable

 

Rb109

Very low

Large

White

waxy

intermediate

Dimple

circular

White

Absent

Single

Freely threshable

 

Rb111

Very low

small

White

waxy

intermediate

Plump

Narrow elliptic

Red

Absent

Single

Partly threshable

 

Ba118

Very low

Large

yellow

waxy

Intermediate

Dimple

circular

White

Absent

Single

Freely threshable

 

Ba119

Very low

Large

White

waxy

Intermediate

Dimple

elliptic

White

Absent

Single

Freely threshable

 

Ba120

Very low

Large

White

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Ba121

Very low

small

White

sugar

Completely starch

Plump

circular

White

Absent

Single

Difficult threshable

 

Assosa-1

Low

Medium

White

sugar

Mostly starch

Plump

circular

White

Absent

Single

Freely threshable

 

Adukara

Very low

Medium

White

sugar

Mostly starch

Plump

circular

Red

Absent

Single

Partly threshable

 

 

Fig. 1. Photo shows different caryopsis grain colors from  the collected land race.

 

REFERENCE

1.   Poehlman, J.andD. Sleper. (1995). Methods in plant breeding. Breeding Field Crops: 172-4.

2.   Doggett, H., ,. (1988). Sorghum Long Mans. 2nd (ed.). Green and Co ltd. London. P, 40-41.

3.   Ringo, J.,A. Onkware,M. Mgonja,S. Deshpande,A. Rathore, et al. (2015). Heterosis for yield and its components in sorghum ('Sorghum bicolor'L. Moench) hybrids in dry lands and sub-humid environments of East Africa. Australian Journal of Crop Science, 9(1).

4.   Gudu, S.,E. Ouma,A. Onkware,E. Too,B. Were, et al, Year. Preliminary Participatory On-farm Sorghum variety selection for tolerance to drought, soil acidity and Striga in Western Kenya. In: (Ed.)^(Eds.), pp.

5.   Taye, T.,E.S. Mace,I.D. GodwinandD.R. Jordan, (2016). Heterosis in locally adapted sorghum genotypes and potential of hybrids for increased productivity in contrasting environments in Ethiopia. 479-89 pp.

6.   Duncan, R.,J. DahlbergandM. Spinks. (1996). International Activities in Sorghum Germplasm Acquisition during the Past Thirty‐Five years. International Germplasm Transfer: Past and Present, 23: 115-34.

7.   Berg, T. (2009). Landraces and folk varieties: a conceptual reappraisal of terminology. Euphytica, 166(3): 423-30.

8.   Upadhyaya, H.,C. GowdaandD. Sastry. (2008). Plant genetic resources management: collection, characterization, conservation and utilization. Journal of SAT Agricultural Research, 6: 16pp.

9.   Harlan, J.R.andJ.M. de Wet. (1972). A simplified classification of cultivated sorghum 1. Crop science, 12(2): 172-6.

10. Vavilov, N.I. (1951). The origin, variation, immunity and breeding of cultivated plants. Edn.: LWW

11. Tesema, T., (2018). Economic Efficiency of Smallholder Farmers in Maize Production in Gudeya Bila District, Oromia National Regional State, Ethiopia

12. Huang, Y. (2004, January). Evaluation of genetic diversity in sorghum germplasm using molecular markers. In International Plant & Animal Genome XII Conference, San Diego, CA. Poster (Vol. 265, p. 138).

13. Dahlberg, J.,X. Zhang,G. HartandJ. Mullet. (2002). Comparative assessment of variation among sorghum germplasm accessions using seed morphology and RAPD measurements. Crop Science, 42(1): 291-6.

14. Ferreira, M.E. (2006). Molecular analysis of gene banks for sustainable conservation and increased use of crop genetic resources. The role of biotechnology in exploring and protecting agricultural genetic resources. Food and Agriculture Organization of the United Nations, Rome, Italy: 121-8.

15. Sergio, L., & Gianni, B. (2005, March). Molecular markers based analysis for crop germplasm preservation. In FAO Meeting on the role of biotechnology for the characterisation and conservation of crops, forestry, animal and fishery genetic resources, Turin, Italy (Vol. 57).

16. Reddy, B.,P. Rao,U. Deb,J. Stenhouse,B. Ramaiah, et al. (2004). Global sorghum genetic enhancement processes at ICRISAT. Sorghum genetic enhancement: research process, dissemination and impacts, 1: 64-101.

17. Prajapati, D.,S. Pahuja,N. VermaandS. Chaudhary. (2018). Morphological characterization of sorghum [Sorghum bicolor (L.) Moench] germplasm for DUS traits. International Journal of Current Microbiology and Applied Sciences, 7(2): 2058-71.

18. Ayyangar, G. N., Rao, V. P., Nambiar, A. K., & Ponnaiah, B. W. X. (1937, January). The occurrence and inheritance of waxy bloom on sorghum. In Proceedings of the Indian Academy of Sciences-Section B (Vol. 5, No. 1, pp. 4-15). Springer India.

19. Dawson, S.M. (1913). A Confederate Girl's Diary. Edn.: Lulu. com

20. Brown, P.,P. Klein,E. Bortiri,C. Acharya,W. Rooney, et al. (2006). Inheritance of inflorescence architecture in sorghum. Theoretical and applied genetics, 113(5): 931-42.

21. Adeyanju, A.,R. PerumalandT. Tesso. (2015). Genetic analysis of threshability in grain sorghum [Sorghum bicolor (L.) Moench]. Plant Breeding, 134(2): 148-55. 

Tao, Y.,Y. Trusov,X. Zhao,X. Wang,A.W. Cruickshank, et al. (2021). Manipulating assimilate availability provides insight into the genes controlling grain size in sorghum. The Plant Journal..

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